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Expression pattern of TLR-2 in the cycle of seminiferous epithelium. Anti-TLR-2 <t>(NB100-56720,</t> Novus, 1/50) primary antibody was used for detection of cells expressing TLR-2. Immune-positive cells expressing TLR-2 appear brown in color. TLR-2 is expressed only in the nuclei and acrosome of elongated spermatids. Please note that PAS staining on the corresponding sequential section (Supplementary Fig. ) indicates that TLR-2 is expressed at early stage of spermatogenesis. Arrow heads indicate immunopositivity at the acrosome. eSt elongated spermatid, Ley Leydig cell, rSt round spermatid, *spermatocytes, and the arrow indicates spermatogonia
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Expression pattern of TLR-2 in the cycle of seminiferous epithelium. Anti-TLR-2 <t>(NB100-56720,</t> Novus, 1/50) primary antibody was used for detection of cells expressing TLR-2. Immune-positive cells expressing TLR-2 appear brown in color. TLR-2 is expressed only in the nuclei and acrosome of elongated spermatids. Please note that PAS staining on the corresponding sequential section (Supplementary Fig. ) indicates that TLR-2 is expressed at early stage of spermatogenesis. Arrow heads indicate immunopositivity at the acrosome. eSt elongated spermatid, Ley Leydig cell, rSt round spermatid, *spermatocytes, and the arrow indicates spermatogonia
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Expression pattern of TLR-2 in the cycle of seminiferous epithelium. Anti-TLR-2 <t>(NB100-56720,</t> Novus, 1/50) primary antibody was used for detection of cells expressing TLR-2. Immune-positive cells expressing TLR-2 appear brown in color. TLR-2 is expressed only in the nuclei and acrosome of elongated spermatids. Please note that PAS staining on the corresponding sequential section (Supplementary Fig. ) indicates that TLR-2 is expressed at early stage of spermatogenesis. Arrow heads indicate immunopositivity at the acrosome. eSt elongated spermatid, Ley Leydig cell, rSt round spermatid, *spermatocytes, and the arrow indicates spermatogonia
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Expression pattern of TLR-2 in the cycle of seminiferous epithelium. Anti-TLR-2 <t>(NB100-56720,</t> Novus, 1/50) primary antibody was used for detection of cells expressing TLR-2. Immune-positive cells expressing TLR-2 appear brown in color. TLR-2 is expressed only in the nuclei and acrosome of elongated spermatids. Please note that PAS staining on the corresponding sequential section (Supplementary Fig. ) indicates that TLR-2 is expressed at early stage of spermatogenesis. Arrow heads indicate immunopositivity at the acrosome. eSt elongated spermatid, Ley Leydig cell, rSt round spermatid, *spermatocytes, and the arrow indicates spermatogonia
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Expression pattern of TLR-2 in the cycle of seminiferous epithelium. Anti-TLR-2 <t>(NB100-56720,</t> Novus, 1/50) primary antibody was used for detection of cells expressing TLR-2. Immune-positive cells expressing TLR-2 appear brown in color. TLR-2 is expressed only in the nuclei and acrosome of elongated spermatids. Please note that PAS staining on the corresponding sequential section (Supplementary Fig. ) indicates that TLR-2 is expressed at early stage of spermatogenesis. Arrow heads indicate immunopositivity at the acrosome. eSt elongated spermatid, Ley Leydig cell, rSt round spermatid, *spermatocytes, and the arrow indicates spermatogonia
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Expression pattern of TLR-2 in the cycle of seminiferous epithelium. Anti-TLR-2 <t>(NB100-56720,</t> Novus, 1/50) primary antibody was used for detection of cells expressing TLR-2. Immune-positive cells expressing TLR-2 appear brown in color. TLR-2 is expressed only in the nuclei and acrosome of elongated spermatids. Please note that PAS staining on the corresponding sequential section (Supplementary Fig. ) indicates that TLR-2 is expressed at early stage of spermatogenesis. Arrow heads indicate immunopositivity at the acrosome. eSt elongated spermatid, Ley Leydig cell, rSt round spermatid, *spermatocytes, and the arrow indicates spermatogonia
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anti-tlr-3 nb100-56571  (Novus Biologicals)
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Novus Biologicals anti-tlr-3 nb100-56571
Expression pattern of TLR-2 in the cycle of seminiferous epithelium. Anti-TLR-2 <t>(NB100-56720,</t> Novus, 1/50) primary antibody was used for detection of cells expressing TLR-2. Immune-positive cells expressing TLR-2 appear brown in color. TLR-2 is expressed only in the nuclei and acrosome of elongated spermatids. Please note that PAS staining on the corresponding sequential section (Supplementary Fig. ) indicates that TLR-2 is expressed at early stage of spermatogenesis. Arrow heads indicate immunopositivity at the acrosome. eSt elongated spermatid, Ley Leydig cell, rSt round spermatid, *spermatocytes, and the arrow indicates spermatogonia
Anti Tlr 3 Nb100 56571, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Expression pattern of TLR-2 in the cycle of seminiferous epithelium. Anti-TLR-2 (NB100-56720, Novus, 1/50) primary antibody was used for detection of cells expressing TLR-2. Immune-positive cells expressing TLR-2 appear brown in color. TLR-2 is expressed only in the nuclei and acrosome of elongated spermatids. Please note that PAS staining on the corresponding sequential section (Supplementary Fig. ) indicates that TLR-2 is expressed at early stage of spermatogenesis. Arrow heads indicate immunopositivity at the acrosome. eSt elongated spermatid, Ley Leydig cell, rSt round spermatid, *spermatocytes, and the arrow indicates spermatogonia

Journal: Histochemistry and Cell Biology

Article Title: Stage-specific expression of Toll-like receptors in the seminiferous epithelium of mouse testis

doi: 10.1007/s00418-024-02310-z

Figure Lengend Snippet: Expression pattern of TLR-2 in the cycle of seminiferous epithelium. Anti-TLR-2 (NB100-56720, Novus, 1/50) primary antibody was used for detection of cells expressing TLR-2. Immune-positive cells expressing TLR-2 appear brown in color. TLR-2 is expressed only in the nuclei and acrosome of elongated spermatids. Please note that PAS staining on the corresponding sequential section (Supplementary Fig. ) indicates that TLR-2 is expressed at early stage of spermatogenesis. Arrow heads indicate immunopositivity at the acrosome. eSt elongated spermatid, Ley Leydig cell, rSt round spermatid, *spermatocytes, and the arrow indicates spermatogonia

Article Snippet: Anti-TLR-1 (B-23, Sc-130896, Santa Cruz Biotechnology, 1/50), anti-TLR 2 (NB100-56720, Novus, 1/50), anti-TLR-3 (NB100-56571, Novus, 1/50), anti-TLR-4 (NB100- 56,566, Novus, 1/50), anti-TLR-5 (H-127, Sc-10742, Santa Cruz Biotechnology, 1/50), anti-TLR-6 (NBP1-54,336, Novus, 1/50), anti-TLR-7 (NB100-56682, Novus, 1/50), anti-TLR-8 (NBP2- 24,917, Novus, 1/50), anti-TLR-9 (NBP2-24,729, Novus, 1/50), anti-TLR-11 (NBP1-77,204, Novus, 1/50), anti-TLR-12 (NBP2-24,833, Novus, 1/50), and anti-TLR-13 (NBP2-24,539, Novus, 1/50) were used as primary antibodies.

Techniques: Expressing, Staining

Expression pattern of TLR-3 in the cycle of seminiferous epithelium. Anti-TLR-3 (NB100-56571, Novus, 1/50) primary antibody was used for detection of cells expressing TLR-3. Immune-positive cells expressing TLR-3 ( a , b , c ) appear brown in color. TLR-3 is expressed in spermatocytes, round and elongated spermatids, endosomal compartments, and acrosomes ( a , b , c ). Please note that PAS staining on the corresponding sequential section (Supplementary Fig. ) indicates that TLR-3 is expressed at early, middle, and late stages of spermatogenesis. Arrow heads indicate immunopositivity at the acrosome. eSt elongated spermatid, Ec endosomal compartment, rSt round spermatid, *spermatocytes, and arrows indicate spermatogonia

Journal: Histochemistry and Cell Biology

Article Title: Stage-specific expression of Toll-like receptors in the seminiferous epithelium of mouse testis

doi: 10.1007/s00418-024-02310-z

Figure Lengend Snippet: Expression pattern of TLR-3 in the cycle of seminiferous epithelium. Anti-TLR-3 (NB100-56571, Novus, 1/50) primary antibody was used for detection of cells expressing TLR-3. Immune-positive cells expressing TLR-3 ( a , b , c ) appear brown in color. TLR-3 is expressed in spermatocytes, round and elongated spermatids, endosomal compartments, and acrosomes ( a , b , c ). Please note that PAS staining on the corresponding sequential section (Supplementary Fig. ) indicates that TLR-3 is expressed at early, middle, and late stages of spermatogenesis. Arrow heads indicate immunopositivity at the acrosome. eSt elongated spermatid, Ec endosomal compartment, rSt round spermatid, *spermatocytes, and arrows indicate spermatogonia

Article Snippet: Anti-TLR-1 (B-23, Sc-130896, Santa Cruz Biotechnology, 1/50), anti-TLR 2 (NB100-56720, Novus, 1/50), anti-TLR-3 (NB100-56571, Novus, 1/50), anti-TLR-4 (NB100- 56,566, Novus, 1/50), anti-TLR-5 (H-127, Sc-10742, Santa Cruz Biotechnology, 1/50), anti-TLR-6 (NBP1-54,336, Novus, 1/50), anti-TLR-7 (NB100-56682, Novus, 1/50), anti-TLR-8 (NBP2- 24,917, Novus, 1/50), anti-TLR-9 (NBP2-24,729, Novus, 1/50), anti-TLR-11 (NBP1-77,204, Novus, 1/50), anti-TLR-12 (NBP2-24,833, Novus, 1/50), and anti-TLR-13 (NBP2-24,539, Novus, 1/50) were used as primary antibodies.

Techniques: Expressing, Staining

Expression pattern of TLR-4 in the cycle of seminiferous epithelium. Anti-TLR-4 (NB100-56566, Novus, 1/50) primary antibody was used for detection of cells expressing TLR-4. Immune-positive cells expressing TLR-4 ( a , b , c ) appear brown in color. TLR-4 is expressed only in elongated spermatids and residual bodies ( a , b , c ). Immune-positive residual bodies are located mainly at the luminal surface of seminiferous of epithelium ( b , c ). Please also note the presence of an immune-positive residual body near the nucleus of a Sertoli cell (thick arrow, b ) and another one close to the basal region of seminiferous of epithelium (thick arrow, c ). Please note that PAS staining on the corresponding sequential section (Supplementary Fig. ) indicates that TLR-4 is expressed at early and middle stages of spermatogenesis. eSt elongated spermatid, Rb residual body, rSt round spermatid, Ser Sertoli cell, *spermatocytes, and thin arrows indicate spermatogonia

Journal: Histochemistry and Cell Biology

Article Title: Stage-specific expression of Toll-like receptors in the seminiferous epithelium of mouse testis

doi: 10.1007/s00418-024-02310-z

Figure Lengend Snippet: Expression pattern of TLR-4 in the cycle of seminiferous epithelium. Anti-TLR-4 (NB100-56566, Novus, 1/50) primary antibody was used for detection of cells expressing TLR-4. Immune-positive cells expressing TLR-4 ( a , b , c ) appear brown in color. TLR-4 is expressed only in elongated spermatids and residual bodies ( a , b , c ). Immune-positive residual bodies are located mainly at the luminal surface of seminiferous of epithelium ( b , c ). Please also note the presence of an immune-positive residual body near the nucleus of a Sertoli cell (thick arrow, b ) and another one close to the basal region of seminiferous of epithelium (thick arrow, c ). Please note that PAS staining on the corresponding sequential section (Supplementary Fig. ) indicates that TLR-4 is expressed at early and middle stages of spermatogenesis. eSt elongated spermatid, Rb residual body, rSt round spermatid, Ser Sertoli cell, *spermatocytes, and thin arrows indicate spermatogonia

Article Snippet: Anti-TLR-1 (B-23, Sc-130896, Santa Cruz Biotechnology, 1/50), anti-TLR 2 (NB100-56720, Novus, 1/50), anti-TLR-3 (NB100-56571, Novus, 1/50), anti-TLR-4 (NB100- 56,566, Novus, 1/50), anti-TLR-5 (H-127, Sc-10742, Santa Cruz Biotechnology, 1/50), anti-TLR-6 (NBP1-54,336, Novus, 1/50), anti-TLR-7 (NB100-56682, Novus, 1/50), anti-TLR-8 (NBP2- 24,917, Novus, 1/50), anti-TLR-9 (NBP2-24,729, Novus, 1/50), anti-TLR-11 (NBP1-77,204, Novus, 1/50), anti-TLR-12 (NBP2-24,833, Novus, 1/50), and anti-TLR-13 (NBP2-24,539, Novus, 1/50) were used as primary antibodies.

Techniques: Expressing, Staining

Expression pattern of TLR-2 in the cycle of seminiferous epithelium. Anti-TLR-2 (NB100-56720, Novus, 1/50) primary antibody was used for detection of cells expressing TLR-2. Immune-positive cells expressing TLR-2 appear brown in color. TLR-2 is expressed only in the nuclei and acrosome of elongated spermatids. Please note that PAS staining on the corresponding sequential section (Supplementary Fig. ) indicates that TLR-2 is expressed at early stage of spermatogenesis. Arrow heads indicate immunopositivity at the acrosome. eSt elongated spermatid, Ley Leydig cell, rSt round spermatid, *spermatocytes, and the arrow indicates spermatogonia

Journal: Histochemistry and Cell Biology

Article Title: Stage-specific expression of Toll-like receptors in the seminiferous epithelium of mouse testis

doi: 10.1007/s00418-024-02310-z

Figure Lengend Snippet: Expression pattern of TLR-2 in the cycle of seminiferous epithelium. Anti-TLR-2 (NB100-56720, Novus, 1/50) primary antibody was used for detection of cells expressing TLR-2. Immune-positive cells expressing TLR-2 appear brown in color. TLR-2 is expressed only in the nuclei and acrosome of elongated spermatids. Please note that PAS staining on the corresponding sequential section (Supplementary Fig. ) indicates that TLR-2 is expressed at early stage of spermatogenesis. Arrow heads indicate immunopositivity at the acrosome. eSt elongated spermatid, Ley Leydig cell, rSt round spermatid, *spermatocytes, and the arrow indicates spermatogonia

Article Snippet: Anti-TLR-1 (B-23, Sc-130896, Santa Cruz Biotechnology, 1/50), anti-TLR 2 (NB100-56720, Novus, 1/50), anti-TLR-3 (NB100-56571, Novus, 1/50), anti-TLR-4 (NB100- 56,566, Novus, 1/50), anti-TLR-5 (H-127, Sc-10742, Santa Cruz Biotechnology, 1/50), anti-TLR-6 (NBP1-54,336, Novus, 1/50), anti-TLR-7 (NB100-56682, Novus, 1/50), anti-TLR-8 (NBP2- 24,917, Novus, 1/50), anti-TLR-9 (NBP2-24,729, Novus, 1/50), anti-TLR-11 (NBP1-77,204, Novus, 1/50), anti-TLR-12 (NBP2-24,833, Novus, 1/50), and anti-TLR-13 (NBP2-24,539, Novus, 1/50) were used as primary antibodies.

Techniques: Expressing, Staining

Expression pattern of TLR-3 in the cycle of seminiferous epithelium. Anti-TLR-3 (NB100-56571, Novus, 1/50) primary antibody was used for detection of cells expressing TLR-3. Immune-positive cells expressing TLR-3 ( a , b , c ) appear brown in color. TLR-3 is expressed in spermatocytes, round and elongated spermatids, endosomal compartments, and acrosomes ( a , b , c ). Please note that PAS staining on the corresponding sequential section (Supplementary Fig. ) indicates that TLR-3 is expressed at early, middle, and late stages of spermatogenesis. Arrow heads indicate immunopositivity at the acrosome. eSt elongated spermatid, Ec endosomal compartment, rSt round spermatid, *spermatocytes, and arrows indicate spermatogonia

Journal: Histochemistry and Cell Biology

Article Title: Stage-specific expression of Toll-like receptors in the seminiferous epithelium of mouse testis

doi: 10.1007/s00418-024-02310-z

Figure Lengend Snippet: Expression pattern of TLR-3 in the cycle of seminiferous epithelium. Anti-TLR-3 (NB100-56571, Novus, 1/50) primary antibody was used for detection of cells expressing TLR-3. Immune-positive cells expressing TLR-3 ( a , b , c ) appear brown in color. TLR-3 is expressed in spermatocytes, round and elongated spermatids, endosomal compartments, and acrosomes ( a , b , c ). Please note that PAS staining on the corresponding sequential section (Supplementary Fig. ) indicates that TLR-3 is expressed at early, middle, and late stages of spermatogenesis. Arrow heads indicate immunopositivity at the acrosome. eSt elongated spermatid, Ec endosomal compartment, rSt round spermatid, *spermatocytes, and arrows indicate spermatogonia

Article Snippet: Anti-TLR-1 (B-23, Sc-130896, Santa Cruz Biotechnology, 1/50), anti-TLR 2 (NB100-56720, Novus, 1/50), anti-TLR-3 (NB100-56571, Novus, 1/50), anti-TLR-4 (NB100- 56,566, Novus, 1/50), anti-TLR-5 (H-127, Sc-10742, Santa Cruz Biotechnology, 1/50), anti-TLR-6 (NBP1-54,336, Novus, 1/50), anti-TLR-7 (NB100-56682, Novus, 1/50), anti-TLR-8 (NBP2- 24,917, Novus, 1/50), anti-TLR-9 (NBP2-24,729, Novus, 1/50), anti-TLR-11 (NBP1-77,204, Novus, 1/50), anti-TLR-12 (NBP2-24,833, Novus, 1/50), and anti-TLR-13 (NBP2-24,539, Novus, 1/50) were used as primary antibodies.

Techniques: Expressing, Staining

Expression pattern of TLR-4 in the cycle of seminiferous epithelium. Anti-TLR-4 (NB100-56566, Novus, 1/50) primary antibody was used for detection of cells expressing TLR-4. Immune-positive cells expressing TLR-4 ( a , b , c ) appear brown in color. TLR-4 is expressed only in elongated spermatids and residual bodies ( a , b , c ). Immune-positive residual bodies are located mainly at the luminal surface of seminiferous of epithelium ( b , c ). Please also note the presence of an immune-positive residual body near the nucleus of a Sertoli cell (thick arrow, b ) and another one close to the basal region of seminiferous of epithelium (thick arrow, c ). Please note that PAS staining on the corresponding sequential section (Supplementary Fig. ) indicates that TLR-4 is expressed at early and middle stages of spermatogenesis. eSt elongated spermatid, Rb residual body, rSt round spermatid, Ser Sertoli cell, *spermatocytes, and thin arrows indicate spermatogonia

Journal: Histochemistry and Cell Biology

Article Title: Stage-specific expression of Toll-like receptors in the seminiferous epithelium of mouse testis

doi: 10.1007/s00418-024-02310-z

Figure Lengend Snippet: Expression pattern of TLR-4 in the cycle of seminiferous epithelium. Anti-TLR-4 (NB100-56566, Novus, 1/50) primary antibody was used for detection of cells expressing TLR-4. Immune-positive cells expressing TLR-4 ( a , b , c ) appear brown in color. TLR-4 is expressed only in elongated spermatids and residual bodies ( a , b , c ). Immune-positive residual bodies are located mainly at the luminal surface of seminiferous of epithelium ( b , c ). Please also note the presence of an immune-positive residual body near the nucleus of a Sertoli cell (thick arrow, b ) and another one close to the basal region of seminiferous of epithelium (thick arrow, c ). Please note that PAS staining on the corresponding sequential section (Supplementary Fig. ) indicates that TLR-4 is expressed at early and middle stages of spermatogenesis. eSt elongated spermatid, Rb residual body, rSt round spermatid, Ser Sertoli cell, *spermatocytes, and thin arrows indicate spermatogonia

Article Snippet: Anti-TLR-1 (B-23, Sc-130896, Santa Cruz Biotechnology, 1/50), anti-TLR 2 (NB100-56720, Novus, 1/50), anti-TLR-3 (NB100-56571, Novus, 1/50), anti-TLR-4 (NB100- 56,566, Novus, 1/50), anti-TLR-5 (H-127, Sc-10742, Santa Cruz Biotechnology, 1/50), anti-TLR-6 (NBP1-54,336, Novus, 1/50), anti-TLR-7 (NB100-56682, Novus, 1/50), anti-TLR-8 (NBP2- 24,917, Novus, 1/50), anti-TLR-9 (NBP2-24,729, Novus, 1/50), anti-TLR-11 (NBP1-77,204, Novus, 1/50), anti-TLR-12 (NBP2-24,833, Novus, 1/50), and anti-TLR-13 (NBP2-24,539, Novus, 1/50) were used as primary antibodies.

Techniques: Expressing, Staining